ORIGINAL ARTICLE |
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Year : 2014 | Volume
: 30
| Issue : 2 | Page : 126-131 |
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Morphological and electron microscopic effects of mitomycin C inhalation in experimentally induced laryngeal fibrosis in dogs
Mohamed T El Tabbakh1, Mohsen Mohamed Hassan Mostafa Zagholol2, Adel R. Hinnis3, Zinab Abdel-Hady4
1 Otolaryngology - Head and Neck Surgery Department, Suez Canal University, Ismailia, Egypt 2 Surgery, Radiology and Anesthesiology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt 3 Pathology Department, Suez Canal University, Ismailia, Egypt 4 Histology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
Correspondence Address:
Mohamed T El Tabbakh Otolaryngology - Head and Neck Surgery Department, Faculty of Medicine, Suez Canal University, 41522 Egypt
Source of Support: None, Conflict of Interest: None | Check |
DOI: 10.4103/1012-5574.133214
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Purpose
This study was carried out to investigate the morphological and ultrastructural effects of mitomycin C in the inhalational form on induced laryngeal tissue injuries and fibrosis.
Materials and methods
This study was carried out on 17 cross-breed clinically healthy dogs. Three dogs were included in a normal control group. The rest of the 14 dogs were subjected to induction of laryngeal fibrosis and assigned randomly to two equal groups: one treated with inhalational mitomycin C and the other was an untreated control group. Tissue biopsies were studied after H&E and Masson's trichrome stains, whereas other pieces were examined by electron microscopy.
Results
Epithelial ulceration with marked inflammatory infiltration, edema, and congestion was evident in all experimented dogs. There was a significant difference in epithelial hyperplasia between the treated and the untreated groups; it was markedly reduced in the treated group. The untreated group showed extensive well-organized fibrosis that was significantly increased compared with the other groups. Results were confirmed by electron microscopy.
Conclusion
Inhalation of mitomycin C has a potent antifibrotic effect through decreasing the activity of fibroblast evident by the decrease in both the size of fibroblast and the density of the intrafibroblast endoplasmic reticulum. |
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